Journal: BMC Infectious Diseases

Article Title: Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting

doi: 10.1186/1471-2334-12-10

Figure Lengend Snippet: IFN-γ levels (pg/ml) elicited by classical TB antigens in 23 TB patients (TB) and 20 household contacts (HHC) . Responses in TB cases are indicated by open symbols and those in household contacts by closed symbols. Error bars represent the median. Ag85A/B = Ag85A and Ag85B tested together, HSP = heat shock protein, PHA = Phytohaemagglutinin.

Article Snippet: Plates were then washed and 1 μg/ml (100 μl) of the biotinylated mouse-anti-human IFN-γ detection antibody (BD PharmingenTM) added.

Techniques:

Journal: BMC Infectious Diseases

Article Title: Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting

doi: 10.1186/1471-2334-12-10

Figure Lengend Snippet: Number of inclusions of antigens into the 10 general discriminant analysis models that most accurately predicted the presence or absence of TB disease . PHA was evaluated in all study participants as a positive control, and appeared in one of the models because IFN-γ data was analyzed in a blinded manner.

Article Snippet: Plates were then washed and 1 μg/ml (100 μl) of the biotinylated mouse-anti-human IFN-γ detection antibody (BD PharmingenTM) added.

Techniques: Positive Control